LncRNA Information
ID EL1517 Name ZFAS1 Aliases C20orf199; HSUP1; HSUP2; NCRNA00275; ZNFX1-AS1
Species Homo sapiens Chromosome 20 Start site 49278178
End site 49295738 Chain plus Exon NO. 5
Assembly Ensembl Release 89 Class antisense NCBI accession NR_003604, NR_036658, NR_003606, NR_003605, NR_036659
Ensembl ENSG00000177410 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
breast cancer microarray, Northern blot, ISH etc. breast cancer tissue down-regulated expression SNORD-host RNA Zfas1 is a regulator of mammary development and a potential marker for breast cancer.ZFAS1 is highly expressed in the mammary gland and is down-regulated in breast tumors compared to normal tissue. ZFAS1 is a putative tumor suppressor gene. 21460236 LncRNADisease Lnc2Cancer
ductal carcinoma N/A N/A N/A expression Human ZFAS1 levels are reduced in ductal carcinoma relative to normal breast tissue. 21460236 LncRNADisease Lnc2Cancer
colorectal cancer qPCR, knockdown, Western blot, Northern blot, RIP, RNA pull-down assay etc. CRC tissue, cell lines ( HCT116+/+ (p53 wild type), HCT116-/- (p53 knockout), HT-29, DLD-1(p53241F), Colo-206, CaCO-2, SW-837, SW-620) up-regulated interaction We determined expression of 83 long non-coding RNAs (lncRNAs) and identified ZFAS1 to be significantly up-regulated in colorectal cancer (CRC) tissue. We observed significant increase in p53 levels and PARP cleavage in CRC cell lines after ZFAS1 silencing indicating increase in apoptosis. Our data suggest that ZFAS1 may function as oncogene in CRC by two main actions: (i) via destabilization of p53 and through (ii) interaction with CDK1/cyclin B1 complex leading to cell cycle progression and inhibition of apoptosis. 26506418 Lnc2Cancer
hepatocelluar carcinoma qPCR, Luciferase reporter assay, RIP etc. HCC tissue, cell lines (Huh7, HepG2, etc.) up-regulated interaction ZFAS1 functions as an oncogene in HCC progression by binding miR-150 and abrogating its tumor suppressive function in this setting. miR-150 repressed HCC cell invasion by inhibiting ZEB1 and the matrix metalloproteinases MMP14 and MMP16. Conversely, ZFAS1 activated ZEB1, MMP14 and MMP16 expression, inhibiting these effects of miR-150. 26069248 Lnc2Cancer
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
ZEB1, MMP14, and MMP16 RNA-DNA regulation ZFAS1 activated ZEB1, MMP14, and MMP16 expression, inhibiting these effects of miR-150. 26069248
miR-150 RNA-RNA binding ZFAS1 functions as an oncogene in HCC progression by binding miR-150 and abrogating its tumor-suppressive function in this setting. 26069248
p53 RNA-Protein regulation destabilization of p53 26506418
CDK1 RNA-Protein regulation Interaction with CDK1/cyclin B1 complex leading to cell cycle progression and inhibition of apoptosis. 26506418