hepatocelluar carcinoma |
microarray, qPCR, Northern blot, FISH etc. |
cell lines (Hep3B, Huh7, and PLC) |
up-regulated |
interaction |
Here, using transcriptome microarray analysis, we identified a long noncoding RNA (lncRNA) termed lncTCF7 that is highly expressed in HCC tumors and liver CSCs. Mechanistically, lncTCF7 recruits the SWI/SNF complex to the promoter of TCF7 to regulate its expression, leading to activation of Wnt signaling. Our data suggest that lncTCF7-mediated Wnt signaling primes liver CSC self-renewal and tumor propagation. |
25842979 |
Lnc2Cancer
|
hepatocelluar carcinoma |
qPCR, Western blot, Luciferase reporter assay etc. |
HCC cell lines (SK-Hep-1, BEL-7402) |
up-regulated |
interaction |
We demonstrate that IL-6 could induce lncTCF7 expression in a time- and dose-dependent manner, and we showed that IL-6 transcriptionally activated the expression of lncTCF7 in HCC cells by activating STAT3, a transcription activator which binds to promoter regions of lncTCF7. Furthermore, knocking-down STAT3 and inhibiting STAT3 activation reduced lncTCF7 expression. Importantly, RNA interference-based attenuation of lncTCF7 prevented IL-6-induced EMT and cell invasion. |
26452542 |
Lnc2Cancer
|