LncRNA Information
ID EL0276 Name CCAT1 Aliases CARLo-5; onco-lncRNA-40
Species Homo sapiens Chromosome 8 Start site 127207866
End site 127219088 Chain minus Exon NO. 2
Assembly Ensembl Release 89 Class lincRNA NCBI accession NR_108049
Ensembl ENSG00000247844 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
acute myeloid leukemia gain- and loss-of-function analysis French-American-British M4 and M5 subtypes of adult AML patients up-regulated N/A repressed monocytic differentiation and promoted cell growth of HL-60 26923190
ovarian cancer microarray, qPCR etc. ovarian cancer tissue, cell lines (SKOV3, SKOV3.ip1 etc.) down-regulated N/A The qPCR results of seven lncRNAs (MALAT1, H19, UCA1, CCAT1, LOC645249, LOC100128881, and LOC100292680) were consistent with the deregulation found by microarray analysis, reflecting the reliability of the microarray data to some extent. 24379988 Lnc2Cancer
gastric cancer N/A gastric cancer (GC) cells and tissues up-regulated N/A CCAT1 regulates miR-490 26825578
gastric cancer N/A N/A N/A expression Level of lncRNA CCAT1 was markedly increased in gastric carcinoma tissue comparing with normal tissue, and overexpressed CCAT1 promoted cancer cell proliferation and migration 24757675 LncRNADisease
gastric cancer N/A N/A N/A expression Another study reported that lncRNA CCAT1 was up-regulated in gastric carcinoma tissues, and its expression was closely related to the transcription factor c-Myc. 24833871 LncRNADisease
gastric cancer qPCR etc. gastric cancer tissue up-regulated expression AGS human gastric carcinoma cell line showed an elevated level of CCAT1 expression. Expression levels of CCAT1 were approximately 10.8 fold higher in GC samples than in samples taken from the negative control group. Interestingly, CCAT1 expression was significantly overexpressed in adjacent normal tissues when compared to the negative control group. Tissues obtained from recurrent GC cases showed the highest expression levels. Expression levels increased with tumor stage, however this did not reach statistical significance. 25561974 Lnc2Cancer
colorectal cancer qPCR etc. CRC tissue up-regulated interaction The expression of IncRNA-CCAT1 in tumor tissue was significantly higher than that in normal para-carcinoma tissue, and the expression level of CCAT1 was significantly correlated with local infiltration depth , tumor staging, vascular invasion and CA19-9 level, it mediates the EMT process of colorectal cancer. 26064266 Lnc2Cancer
hepatocelluar carcinoma qPCR etc. HCC tissue, cell lines (L-02, HepG2, SNU423, SMMC-7721, Hep3B) up-regulated expression The results indicated that the expression of CCAT1 was significantly increased in HCC tissues and cells compared with controls. We also found that the abnormally expressed CCAT1 could promote cell proliferation, migration and invasion. Taken together, our findings demonstrated that the aberrant expression of CCAT1 promotes hepatocellular carcinoma in vitro 26191246 Lnc2Cancer
hepatocelluar carcinoma qPCR etc. Liver cancer cell lines (HepG2, Hep3B, SK-HEP1, SMMC7721, MHCC97-L, MHCC97-H, PLC/PRF/5, HCCLM3) up-regulated expression The results showed that CARLo-5 levels were significantly overexpressed in HCC tissues compared to ANLT. Besides, high expression of CARLo-5 was associated with liver cirrhosis (P = 0.001), tumor number (P < 0.001), vascular invasion (P = 0.001), capsular formation (P = 0.014) and Edmondson-Steiner grade (P < 0.001), which proved that CARLo-5 was an independent risk factor for overall survival and disease-free survival. In addition, in highly metastatic HCC cell lines (HCCLM3 and MHCC97-L), CARLo-5 was up-regulated, but in lowly metastatic HCC cell lines (HepG2, SNU387), it showed down-regulated. Besides, by using gain and loss of function experiments in HCC cell lines (HCCLM3 and HepG2), the results showed that CARLo-5 overexpression significantly enhanced cell proliferation, migration and invasion in vitro. Our study also revealed that CARLo-5 was prominently up-regulated in HCC specimens and its high expression was associated with poor prognosis of HCC patients 26433964 Lnc2Cancer
breast cancer qPCR etc. BC tissue up-regulated expression Expression levels of lncRNA CCAT1 in BC tissues were significantly higher than those in adjacent normal tissues. High expression of lncRNA CCAT1 was associated with differentiation grade, TNM stage, and lymph node metastases. Kaplan-Meier analysis with the log-rank test indicated that high expression of lncRNA CCAT1 had a decreased overall survival and progression-free survival. Multivariable analysis was further identified high expression of lncRNA CCAT1 as an independent prognosis factor for overall survival and progression-free survival. 26464701 Lnc2Cancer
colorectal cancer qPCR, ISH etc. cell lines (SW-480, HT-29, HT29, SW-480 etc.) up-regulated expression Recently, colon cancer associated transcript 1 (CCAT1) lncRNA was found to be expressed in colorectal cancer (CRC) tumors but not in normal tissue. 23416875 LncRNADisease Lnc2Cancer
colon cancer qPCR, ISH etc. colonic adenoma-carcinoma tissue up-regulated N/A CCAT1 is up-regulated across the colon adenoma-carcinoma sequence. This up-regulation is evident in pre-malignant conditions and through all disease stages, including advanced metastatic disease suggesting a role in both tumorigenesis and the metastatic process. 23594791 Lnc2Cancer
colorectal cancer qPCR, knockdown etc. primary prostatecancer tissue up-regulated N/A CARLo-5 is highly expressed in CRC-derived cell lines compared with normal colon-derived fibroblasts and CRC primary tissues compared with their matched normal adjacent tissues (NATs). In addition, CARLo-5 is highly expressed in prostate cancer (PC) tissues compared with their NATs. CARLo-5 is significantly correlated with the rs6983267 allele associated with increased cancer susceptibility. We also found the MYC enhancer region physically interacts with the active regulatory region of the CARLo-5 promoter, suggesting long-range interaction of MYC enhancer with the CARLo-5 promoter regulates CARLo-5 expression. 24594601 Lnc2Cancer
gallbladder cancer qPCR, Luciferase reporter assay, Western blot etc. gallbladder cancer tissue up-regulated expression In this study, we demonstrated that CCAT1 was upregulated in gallbladder cancer (GBC) tissues. CCAT1 silencing downregulated, whereas CCAT1 overexpression enhanced the expression of miRNA-218-5p target gene Bmi1 through competitively 'spongeing' miRNA-218-5p. Our data revealed that CCAT1 knockdown impaired the proliferation and invasiveness of GBC cells, at least in part through affetcing miRNA-218-5p-mediated regulation of Bmi1. Moreover, CCAT1 transcript level was correlated with Bmi1 mRNA level in GBC tissues. 25569100 Lnc2Cancer
gastric cancer qPCR, Western blot, in vitro knockdown etc. gastric cancer tissue up-regulated expression In the present study, a great upregulation of CARLo-5 was observed in gastric cancer compared to paired adjacent normal tissues. Knockdown of CARLo-5 in gastric cancer cell lines significantly inhibited the cell proliferation via inducing G0/G1 cell-cycle arrest and apoptosis. 25674211 Lnc2Cancer
non-small cell lung cancer qPCR, Western blot, knockdown etc. NSCLC tissue, cell lines (A549, SPC-A1, NCI-H1975) up-regulated interaction In the present study, a great upregulation of CARLo-5 was observed in cancer tissues compared to their adjacent normal tissues. Meanwhile, patients with high CARLo-5 expression have significantly poorer prognosis than those with low expression. Inhibition of CARLo-5 by siRNA suppressed the proliferation, migration, and invasion in NSCLC cell lines in vitro. In addition, silencing of CARLo-5 reversed the epithelial-mesenchymal transition in NSCLC cell line. 25129441 Lnc2Cancer
gastric cancer qPCR, Western bolt, knockdown, Luciferase reporter assay, RIP etc. gastric carcinoma tissue, cell lines (AGS, MKN45) up-regulated regulation Long noncoding RNA CCAT1, which could be activated by c-Myc, promotes the progression of gastric carcinoma. 23143645 LncRNADisease Lnc2Cancer
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
miRNA-218-5p RNA-RNA binding these results suggest that CCAT1 is a driver of malignancy, which acts in part through 'spongeing' miRNA-218-5p. 25569100
E-cadherin and N-cadherin RNA-Protein co-expression The expression of IncRNA-CCAT1 in tumor tissue was significantly higher than that in normal para-carcinoma tissue (P < 0.001), and the expression level of CCAT1was significantly correlated with local infiltration depth (P < 0.001), tumor staging (P < 0.001), vascular invasion (P < 0.001) and CA19-9 level (P < 0.001); but not related with age, gender, location of tumor, tumor differentiation level, size of primary lesion and level of CEA (P > 0.05). The expression of E-cadherin in tumor tissues was significantly lower than in normal para-carcinoma tissues (P < 0.001), and the expression of N-cadherin was significantly higher than that in normal para-carcinoma tissues. The decrease in expression of E-cadherin and increase in expression of N-cadherin were significantly correlated with local infiltration depth (P < 0.001), tumor staging (P < 0.001), vascular invasion (P < 0.001), tumor differentiation level (P < 0.001) and CA19-9 level (P < 0.001), however not related with age, gender, tumor location, size of primary lesion and CEA level (P > 0.05). CONCLUSION: CCAT1 plays an important role in the genesis, development, invasion and metastasis; it mediates the EMT process of colorectal cancer; and it's expected to be a new marker and treatment target in colorectal diagnosis and treatment. 26064266
miR-490 RNA-RNA binding CCAT1 regulates miR-490 in gastric cancer (GC) cells. miR-490 can also repress CCAT1 expression. CCAT1 contains a putative miR-490-binding site, and deletion of this binding site abolishes their miR-490 responsiveness. 26825578