LncRNA Information
ID EL0973 Name NEAT1 Aliases LINC00084; NCRNA00084; TncRNA; VINC
Species Homo sapiens Chromosome 11 Start site 65422774
End site 65445540 Chain plus Exon NO. 1
Assembly Ensembl Release 89 Class lincRNA NCBI accession NR_131012, NR_028272
Ensembl ENSG00000245532 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
gastric adenocarcinoma GAC tissues and matched adjacent normal qRT-PCR gastric adenocarcinomas (GACs) up-regulated N/A Expression of NEAT1 lncRNA was enhanced in GACs and influence GAC progression by promoting tumor growth 26911892
gastric cancer gastric cancer samples and cell lines by real-time PCR gastric cancer tissues and cell lines up-regulated N/A LncRNA NEAT1 was overexpressed in gastric cancer tissues and cell lines and corrected with clinical stage, histological type, lymph node metastasis, and distant metastasis 27095450
paraspeckle disintegration Immunoprecipitation of Ribonucleoprotein Complex,knockdown HeLa cell down-regulated mutation Successful removal of MENepsilon/beta by a refined knockdown method resulted in paraspeckle disintegration. Furthermore, the reassembly of paraspeckles disassembled by transcriptional arrest appeared to be unsuccessful in the absence of MENepsilon/beta. 19188602
ovarian cancer knockdown of NEAT1_1 OC patients and OVCAR-3 cell lines up-regulated N/A lncRNA NEAT1, whose expression was collaboratively controlled by HuR and miR-124-3p, could regulate ovarian carcinogenesis 27075229
malignant pleural mesothelioma microarray, qPCR etc. MPM tissue, cell lines (H28, H226, H2052, H2452, MSTO etc.) up-regulated N/A AK130977 and AX746718 were both found to be down-regulated via both microarray and RT-qPCR (AK130977 Microarray (MA) = -5.207, RT-qPCR = -1.6; AX746718 MA = -3.37, RT-qPCR = -4.6), with AK130977 demonstrating fairly small changes using RT-qPCR. Similarly, BX648695, AK129685, EF177379, AK054908, AK130275, AF268386 and NR_003584 all demonstrated consistent up-regulation using both microarrays and RT-qPCR. 23976967 Lnc2Cancer
papillary thyroid carcinoma microarray, qPCR etc. papillary thyroid carcinoma tissue up-regulated expression Expression profiles of five lnc-RNAs (MEG3, HULC, HOTAIR, NEAT1, and MALAT-1) previously shown to be involved in cancer metastasis were detected by qPCR in 5 pairs of papillary thyroid cancer and 11 matched lymph node metastatic tissues. Among the five, MEG3 showed significant down-expression. Overexpression of MEG3 inhibits thyroid cancer cell migration and invasion. 25997963 Lnc2Cancer
prostate cancer microarray, RNA-seq, qPCR, Northern bolt, knockdown etc. prostate cancer tissue, cell lines (LNCaP, CWR22Rv1, PC3 etc.) up-regulated N/A Interestingly, nine out of these thirteen known cancer-related lncRNA showed significantly differential expression between tumor and normal prostate samples. Several lncRNA such as NEAT1, DANCR, HOTTIP, PRINS, and EGOT that have established functions in forming nuclear speckles, in development or in autoimmune disease, but were not previously known to be related to cancer, showed differential expression between tumor and normal prostate samples, suggesting their potential function in prostate cancer. 23728290 Lnc2Cancer
nasopharyngeal carcinoma N/A NPC cell lines and tissues up-regulated N/A significantly upregulated in NPC cell lines and tissues 27020592
frontotemporal lobar degeneration N/A N/A N/A Interaction Analysis of RNA binding by TDP-43 in brains from subjects with FTLD revealed that the greatest increases in binding were to the MALAT1 and NEAT-1 noncoding RNAs. 20581224 LncRNADisease Lnc2Cancer
Huntington's disease N/A N/A N/A expression LncRNAs TUG1 (necessary for retinal development), and NEAT-1 (a structural component of nuclear paraspeckles) are upregulated in HD caudate, while the brain-specific tumor-suppressor MEG3 is downregulated in HD. 23346095 LncRNADisease
AIDS N/A N/A N/A expression We found NEAT-1 to be one of several lncRNAs whose expression is changed by HIV-1 infection, and we have characterized its role in HIV-1 replication. 23362321 LncRNADisease
amyotrophic lateral sclerosis N/A N/A N/A regulation The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis. 23835137 LncRNADisease
intrauterine growth restriction N/A N/A N/A expression The long non-coding RNA NEAT-1 is increased in IUGR placentas, leading to potential new hypotheses of IUGR origin/development. 24280234 LncRNADisease
oral squamous cell carcinoma N/A N/A N/A expression Subsequently, they confirmed that the expression levels of HOTAIR, NEAT-1 and UCA2 in metastasized samples was prominent higher than the non-metastatic samples.? 24817925 LncRNADisease
oral squamous cell carcinoma qPCR etc. OSCC tissues up-regulated N/A We found that most of the selected transcripts (4/6) were upregulated in tumors relative to matched adjacent nonmalignant tissue. One gene, MEG-3, was downregulated in cancer compared with its adjacent nonmalignant tissue. Expression of lncRNA (HOTAIR, NEAT-1 and UCA1) was significantly higher in the samples that subsequently metastasized compared with the non-metastatic samples. By contrast, MEG-3 was downregulated in the metastatic samples. These findings suggest that the detection of lncRNAs in saliva may be used as a noninvasive and rapid diagnostic tool for the diagnosis of oral cancer. 23292713 Lnc2Cancer
non-small cell lung cancer qPCR etc. lung cancer tissue up-regulated expression The relative level of NEAT1 in NSCLC tissues was significantly elevated as compared to that of the adjacent non-cancer lung tissues. NEAT1 expression was positively correlated with patient age, lymphatic metastasis, vascular invasion and clinical TNM stage. lncRNA NEAT1 may act as a oncogene, which plays an important role in the tumorigenesis and deterioration of human NSCLC. 25854373 Lnc2Cancer
hepatocelluar carcinoma qPCR etc. HCC tissue up-regulated interaction Our results revealed that NEAT1 appeared to have higher expression in the HCC tissues, compared with the adjacent non-cancerous liver tissues. High levels of NEAT1 promoted the clinical features of HCC, including the number of tumor nodes, metastasis, clinical TNM stage, the status of portal vein tumor embolus, vaso-invasion and the infiltration of tumor cells. Additionally, high NEAT1 expression levels were significantly associated with the expression level of MDTH, NM23 and MALAT1 26191242 Lnc2Cancer
colorectal cancer qPCR etc. CRC tissue up-regulated expression Results showed that NEAT1 expression in colorectal cancer was up-regulated in 72.0% (172/239) cases compared with corresponding normal counterparts, and related to tumor differentiation, invasion, metastasis and TNM stage. 26314847 Lnc2Cancer
glioma qPCR etc. glioma tissue up-regulated expression In our results, the relative level of NEAT1 expression was higher in cancer tissues compared with adjacent noncancerous tissues (p < 0.001). High NEAT1 expression was observed to be closely correlated with larger tumor size (p = 0.023), higher WHO grade (p = 0.005), and recurrence (p = 0.011). 26582084 Lnc2Cancer
breast cancer qPCR, ISH etc. cell lines (MCF-7, MDA-MB-231, MDAMB-468) up-regulated expression Induction of NEAT1 in hypoxia also leads to accelerated cellular proliferation, improved clonogenic survival and reduced apoptosis, all of which are hallmarks of increased tumorigenesis. Furthermore, in patients with breast cancer, high tumor NEAT1 expression correlates with poor survival, all of which are hallmarks of increased tumorigenesis. 25417700 Lnc2Cancer
esophageal squamous cell carcinoma qPCR, knockdown etc. ESCC tissue, cell lines (SHEE, SHEEC) up-regulated expression We found that the expression of NEAT1 was higher in ESCC tissues and cells compared with the normal counterparts. Pearson analysis showed that elevated NEAT1 levels were extraordinarily correlated with the tumor size (P=0.026), lymph node metastasis (P=0.035) and clinical stage (P=0.004). 26609486 Lnc2Cancer
acute promyelocytic leukemia qPCR, Western blot etc. blood, cell lines (NB4, NB4-R2, U937-PR9) down-regulated expression We found that NEAT1 is significantly repressed in de novo APL samples compared with those of healthy donors. We further provide evidence that NEAT1 expression was repressed by PML-RAR抅 Furthermore, significant NEAT1 upregulation was observed during all-trans retinoic acid (ATRA)-induced NB4 cell differentiation. 25245097 Lnc2Cancer
Burkitt's lymphoma qPCR, Western blot, knockdown etc. blood up-regulated interaction NEAT1 expression levels were validated by qPCR, demonstrating high baseline expression (average Cp = 21.3), and confirming p53-dependent induction . lncRNAs NEAT1 and lincRNA-p21 as novel elements of the p53-dependent DNA damage response machinery in CLL and lymphoma. 25971364 Lnc2Cancer
chronic lymphocytic leukemia qPCR, Western blot, knockdown etc. blood up-regulated expression NEAT1 expression levels were validated by qPCR, demonstrating high baseline expression (average Cp = 21.3), and confirming p53-dependent induction .lncRNAs NEAT1 and lincRNA-p21 as novel elements of the p53-dependent DNA damage response machinery in CLL and lymphoma. 25971364 Lnc2Cancer
lung cancer qRT-PCR lung carcinoma cell lines N/A expression Our data indicate that miR-449a may function as a suppressor of lung cancer, and affects the expression of NEAT1 in lung cancer cells. 25818739
non-small-cell lung cancer qRT-PCR patients suffering from non-small-cell lung cancer (NSCLC) up-regulated expression N/A 26448925
obesity reexpression adipocyte-derived stem cells (ADSCs) isolated from wild-type and microRNA 140 (miR-140) knockout mice N/A expression MiR-140 knockout ADSCs have dramatically decreased adipogenic capabilities associated with downregulation of NEAT1 expression. Reexpression of NEAT1 in miR-140 knockout ADSCs is sufficient to restore their ability to undergo differentiation. 26457124
prostate cancer RNA-seq, qPCR, ChIP etc. prostate cancer tissue, cell lines (LnCaP, PC3) up-regulated expression Among putatively ERa-regulated intergenic long non-coding RNAs (lncRNAs), we identified nuclear enriched abundant transcript 1 (NEAT1) as the most significantly overexpressed lncRNA in prostate cancer. Prostate cancer cells expressing high levels of NEAT1 were recalcitrant to androgen or AR antagonists. NEAT1 drives oncogenic growth by altering the epigenetic landscape of target gene promoters to favour transcription. 25415230 Lnc2Cancer
 


Function (not disease relevant)
Methods Sample/condition Expression pattern Dysfunction type Description PMID Source
EM observation mammalian cells N/A Interaction Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). 25831520
knockdown HeLa epithelial adenocarcinoma,WI-38 normal female diploid fibroblast lung,Human EBV-transformed human lymphoblasts,primary mouse embryo fibroblasts N/A mutation Depletion of NEAT1 RNA via RNAi eradicates paraspeckles, suggesting that it controls sequestration of the paraspeckle proteins PSP1 and p54, factors linked to A-I editing. Unlike overexpression of PSP1, NEAT1 overexpression increases paraspeckle number, and paraspeckles emanate exclusively from the NEAT1 transcription site 19217333
N/A Human cell N/A N/A paraspeckle formation 20211624
N/A N/A N/A expression Biogenesis, metabolism, and functions of lncRNAs are otherwise interconnected with known pathogenic mechanisms 23791884 LncRNADisease
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
PSP-1 RNA-Protein binding The PSP-1 RNA binding domain is required for its colocalization with NEAT1 RNA in paraspeckles, and biochemical analyses support that NEAT1 RNA binds with paraspeckle proteins. 19217333 LncRNADisease
DBHS RNA-Protein binding DBHS protein expression did not vary between the two cellular contexts, instead, paraspeckle induction and increased RNA nuclear retention correlated with the expression of the paraspeckle-specific structural ncRNA species NEAT1. 19720872 LncRNADisease
P54NRB/NONO RNA-Protein binding NEAT1 associates with DBHS proteins in vivo, as immunoprecipitation of PSF/SFPQ, P54NRB/NONO, and PSPC1 all copurify NEAT1 RNA to varying levels. 19720872 LncRNADisease
PSF/SFPQ RNA-Protein binding NEAT1 associates with DBHS proteins in vivo, as immunoprecipitation of PSF/SFPQ, P54NRB/NONO, and PSPC1 all copurify NEAT1 RNA to varying levels. 19720872 LncRNADisease
PSPC1 RNA-Protein binding NEAT1 associates with DBHS proteins in vivo, as immunoprecipitation of PSF/SFPQ, P54NRB/NONO, and PSPC1 all copurify NEAT1 RNA to varying levels. 19720872 LncRNADisease
P54nrb RNA-Protein binding a novel mechanism which is different from that reported 20211624
PSP1 RNA-Protein binding NEAT1 directly interacts with the paraspeckle protein PSP1. NEAT1 knockdown abolishes paraspeckles and relaxes the constraints on export of Alu-containing RNAs into the cytoplasm. 20951849 LncRNADisease
p54nrb RNA-Protein binding In the current model， NEAT1_2 interacts with core paraspeckle proteins PSF and p54nrb, which recruit PSP1 and NEAT1_1 and other associating molecules to the periphery of paraspeckles. 21444682 LncRNADisease
PSF RNA-Protein binding In the current model， NEAT1_2 interacts with core paraspeckle proteins PSF and p54nrb, which recruit PSP1 and NEAT1_1 and other associating molecules to the periphery of paraspeckles. 21444682 LncRNADisease
Proteins of the Drosophila behavior/human splicing RNA-Protein binding Additionally, mammalian DBHS proteins associate with the architectural long noncoding RNA NEAT1 to form paraspeckles, subnuclear bodies that alter gene expression via the nuclear retention of RNA. 22416126 LncRNADisease
miR-449a RNA-RNA regulation Further study of the interaction between miR-449a and NEAT1 show that NEAT1 was up-regulated when cells were transfected with miR-449a inhibitor, and NEAT1 was down-regulated when cells transfected with miR-449a mimics. 25818739
SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes RNA-Protein binding Subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. 25831520
p53 RNA-Protein regulation We used isogenic lymphoma cell line models to prove p53 dependence of NEAT1 and lincRNA-p21. The current work describes the p53-dependent miRNome and identifies lncRNAs NEAT1 and lincRNA-p21 as novel elements of the p53-dependent DNA damage response machinery in CLL and lymphoma. 25971364
MDTH, NM23 and MALAT1 DNA-DNA co-expression high NEAT1 expression levels were significantly associated with the expression level of MDTH, NM23 and MALAT1. 26191242
miR-140 DNA-RNA binding a miR-140 binding site in NEAT1 was identifiedand and mature miR-140 in the nucleus can physically interact with NEAT1, leading to increased NEAT1 expression. 26457124
miR-204/ZEB1 RNA-RNA regulation There was reciprocal repression between NEAT1 and miR-204. 27020592
ZEB1 RNA-Protein regulation ZEB1 was identified as a downstream target of miR-204 and NEAT1 upregulated ZEB1 expression by negatively regulating miR-204 expression. 27020592
HuR RNA-Protein binding NEAT1_1 was stabilized by an RNA-binding protein HuR, but suppressed by miR-124-3p in OC cells. 27075229
miR-124-3p RNA-RNA regulation NEAT1_1 was stabilized by an RNA-binding protein HuR, but suppressed by miR-124-3p in OC cells. 27075229