| RT-PCR |
Nodule formation |
down-regulated |
N/A |
In sections of roots collected 2d post-inoculation, GUS activity was present in dividing cortical cells, indicating that MtENOD40-2 is expressed in cells of the nodule primordium (Fig. 2C). Whole-mount staining for GUS activity of nodules showed that GUS activity is detected near the apex of the nodule and in vascular bundles (Fig. 2D). To localize the site of expression of MtENOD40-2 precisely in the nodule, in situ hybridization using [35S]UTP-labelled antisense MtENOD40-2 RNA was conducted (Fig. 2A, B). This showed that MtENOD40-2 is expressed in cells of the infection zone (Fig. 2B, IZ). Thus, the MtENOD40-2 expression pattern is similar to the MtENOD40-1 expression pattern (Crespi et al., 1994). Furthermore, the GUS expression studies are consistent with the in situ hybridization data, indicating that the 1.8 kb DNA fragment used contains the elements required for the regulation of MtENOD40-2 expression. Based on the combination of expression data and the sequence homology between both genes, it is likely that MtENOD40-2 is functional in nodule initiation and development. |
17452749 |
PLNlncRbase
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