RT-PCR |
Nodule formation |
down-regulated |
N/A |
In M.truncatula, knock-down of MtENOD40-1 expression led to a 50% reduction in the number of nodules. To reduce MtENOD40-1 and MtENOD40-2 gene expression, Agrobacterium rhizogenes-mediated RNAi was applied in M.truncatula hairy roots. To this end, one vector (pRRsil401) was designed that is expected to lead to a reduction in expression of MtENOD40-1, and a second vector (pRRsil402) that is expected to lead to a reduction in MtENOD40-2 expression. To knock-down transcription of both genes simultaneously, a third vector (pRRsil4012) was used. MtENOD40-1 as well as MtENOD40-2 is involved in nodule initiation. On roots,in which the expression of both MtENOD40-1 and MtENOD40-2 is reduced, the average number of nodules per root is 1.5 (75% reduction). Thus knocking down of the expression of both genes has an additive effect, suggesting that MtENOD40 acts in a dose-dependent manner. |
17452749 |
PLNlncRbase
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