LncRNA Information
ID EL0088 Name ASCO-RNA Aliases N/A
Species Arabidopsis thaliana Chromosome N/A Start site N/A
End site N/A Chain N/A Exon NO. N/A
Assembly N/A Class antisense NCBI accession N/A
Ensembl N/A Sequence N/A

Function (not disease relevant)
Methods Sample/condition Expression pattern Dysfunction type Description PMID Source
qRT-PCR leaves up-regulated N/A Double Atnsr mutants and ASCO overexpressors exhibit an altered ability to form LRs after auxin treatment. Interestingly, auxin induces a major change in AS patterns of many genes, a response largely dependent on NSRs. RNA immunoprecipitation assays demonstrate that AtNSRs interact not only with their alternatively spliced mRNA targets but also with the ASCO-RNA in vivo. The ASCO-RNA displaces an AS target from an NSR-containing complex in vitro. Expression of ASCO-RNA(lnc351) in Arabidopsis affects the splicing patterns of several NSR-regulated mRNA targets. Hence, lncRNA can hijack nuclear AS regulators to modulate AS patterns during development. Plants overexpressing the ASCO-RNA showed changes in isoform distribution of the auxin-related protein (At2G33830). (Bardou et al., 2014) Expression of the lncRNA ASCO-RNA do not promote lateral root growth. The lncRNA does not cause NSR relocalization but alters NSR activity through direct binding to NSRs and displacement of them from their mRNA targets. In other words, ASCO-RNA prevents the effects of NSRs on the regulation of alternative splicing in their transcript targets. ASCO-RNA overexpression in live plants duplicates the morphological effects observed in the nsra/nsrbdouble mutant, i.e., a decreased lateral root density when plants are grown on auxin. (Kornblihtt et al., 2014) 25073154, 25073153 PLNlncRbase

Interaction target Level of interaction Type of interaction Description PMID Source
At2G33830 RNA-RNA binding By using an in vitro binding assay for an AS target (the auxinregulated protein gene At2g33830), we showed that incubation with nonlabeled At2g33830 mRNA or the ASCO-RNA significantly diminishes the amount of AS target immunoprecipitated 25073154, 25073153 PLNlncRbase