| RT-PCR |
pollen development |
up-regulated |
N/A |
A 828-bp full-length cDNA of BcMF11, a novel pollen-specific non-coding mRNA-like gene from Chinese cabbage. Antisense RNA transgenic plants displayed decreasing expression of BcMF11 and showed distinct morphological defects. Pollen germination test in vitro and in vivo of the transgenic plants suggested that inhibition of BcMF11 decreased pollen germination efficiency and delayed the pollen tubes’ extension in the style. Under scanning electron microscopy, many shrunken and collapsed pollen grains were detected in the antisense BcMF11 transgenic Chinese cabbage. Further cytological observation revealed abnormal pollen development process in transgenic plants, including delayed degradation of tapetum, asynchronous separation of microspore, and aborted development of pollen grain. These results suggest that BcMF11, as a non-coding RNA, plays an essential role in pollen development and male fertility. RT-PCR and Northern blotting analyses showed that BcMF11 was transcribed at most stages of pollen development. These results suggested that the BcMF11 is a novel non-coding RNA involved in pollen development of Chinese cabbage. (Song et al., 2013) The BcMF11 cDNA has a total length of 828 bp with poly (A) tail. Analysis of the sequence demonstrated that BcMF11 is a novel non-coding RNA which has no prominent open reading frame (ORF) or coding capacity. Transcription analysis indicated that BcMF11 is a novel pollen-specific ncRNA and may be involved in the pollen development of Chinese cabbage. BcMF11 was expressed in developing flower buds, flowers and anthers at a high level, but was not detected in roots, stems, leaves, pistils and developing siliques. Identical expression patterns were also showed in RT-PCR analysis. This indicated BcMF11 is a pollen-preferential expressed gene in Chinese cabbage. (Song et al., 2007) |
23064614, 17207554 |
PLNlncRbase
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