| Northern blot/RT-PCR |
roots |
up-regulated |
N/A |
We also tested the effects of overexpressing a close IPS1 paralog, At4. As with IPS1I, At4 overexpression also resulted in decreased shoot Pi accumulation (Supplementary Fig. 4a online). This finding suggests redundancy between IPS1 and At4 and possibly among the other IPS1 family members, all of which are responsive to Pi starvation and contain a region of miR-399 complementarity (Supplementary Fig. 1). (Franco-Zorrilla et al., 2007) The second regulatory mechanism of lncRNAs in plants might work as a decoy of miRNAs, these kind of lncRNAs exert their functions by binding miRNAs in a target mimicry mechanism to sequestrate the miRNAs’ regulation roles on their target genes, such as lncRNAs IPS1 and at4. IPS1 is complementary to miR399, but contains a mismatch loop which makes it uncleavable when miR399 binding. It is induced by phosphate starvation in plants, and then allows the increased expression of miR399 targets including PHO2, consequently alters shoot phosphate content. IPS1 has many family members in a number of plant species, including At4, At4-1, At4-2 and At4-3 in Arabidopsis thaliana. At4 appears to be functionally redundant with IPS1, overexpression of one of them could result in the same phenotypic change as that when overexpressing both At4 and IPS1. (Zhang et al., 2013) |
17643101, 23726911 |
PLNlncRbase
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