LncRNA Information
ID EL1456 Name WT1-AS Aliases WIT-1; WIT11; WT1AS; WT1-AS
Species Homo sapiens Chromosome 11 Start site 32435518
End site 32458769 Chain plus Exon NO. 8
Assembly Ensembl Release 89 Class antisense NCBI accession NR_120548, NR_120547, NR_023920, NR_120549, NR_120546
Ensembl ENSG00000183242 Sequence

Disease Method Sample Expression pattern Dysfunction type Description PMID Source
Wilms' tumor N/A renal and haematopoietic cells down-regulated expression Both wt1-as and the novel awt1 transcript are imprinted in normal kidney with expression confined to the paternal allele. 14681303
acute myeloid leukemia qPCR etc. bone marrow tissue differential expression epigenetics The incidence of WIT-1 methylation in primary refractory AML was significantly higher than that noted in chemosensitive AML. 10340388 LncRNADisease Lnc2Cancer
acute myeloid leukemia qPCR etc. Bone marrow up-regulated Interaction In AML, there is often abnormal splicing of WT1-AS, which may play a role in the development of this malignancy. 17940140 LncRNADisease Lnc2Cancer
Wilms' tumor qPCR etc. Bone marrow up-regulated Interaction WT1 is a gene that is mutated in Wilms' tumor (WT) and acute myeloid leukaemia (AML) and has an antisense transcript (WT1-AS), which was found to regulate WT1 protein levels. 17940140 LncRNADisease Lnc2Cancer
gastric cancer qPCR etc. gastric cancer tissue, cell lines (SGC7901, BGC823, MKN45, MKN28, NCI-N87, AGS, HS-746T, GES-1) down-regulated expression We found that WT1-AS expression was significantly down-regulated in tumor tissues compared to matched adjacent non-tumor tissues. The WT1-AS expression level was also associated with tumor size and the clinicopathological stage. Cell proliferation, migration, and invasion were inhibited, and the proportion of G0/G1 cells increased when WT1-AS was ectopically-expressed in gastric cancer cells. Furthermore, ectopic expression of WT1-AS was demonstrated to inhibit tumor growth and metastasis in vivo. Finally, we found that WT1-AS overexpression could decrease ERK protein phosphorylation. 26449525 Lnc2Cancer
Wilms' tumor qPCR, Southern blot, knockdown etc. Wilms' tumor tissue differential expression epigenetics A CTCF-binding silencer regulates the imprinted genes AWT1 and WT1-AS and exhibits sequential epigenetic defects during Wilms' tumourigenesis. 17210670 LncRNADisease Lnc2Cancer
hepatocelluar carcinoma qPCR, Western blot, Luciferase reporter assay etc. hepatocellular carcinoma tissue down-regulated interaction WT1-AS expression correlated negatively with WT1 expression in HCC tumor tissue. Kaplan-Meier curve analysis revealed that WT1-AS expression is a reliable indicator of HCC prognosis. The downregulation of WT1 expression by WT1-AS promoted cell apoptosis by suppressing the JAK/STAT3 signaling pathway. Bioinformatics analysis showed that WT1-AS downregulates WT1 by binding to the TATA region of the WT1 promotor. WT1-AS was also able to reverse WT1-mediated resistance to Dox based chemotherapy in HCC cells 26462627 Lnc2Cancer
Wilms' tumor Southern blot analysis, RT-PCR kidney up-regulated epigenetics Wts display hypomethylation and biallelic expression of wt1-as. 10811108

Interaction target Level of interaction Type of interaction Description PMID Source
WT1 RNA-DNA binding WT1-AS downregulates WT1 by binding to the TATA region of the WT1 promotor. 26462627