microarray, RNA interference, RNA-chiP assay, bioinformatics, luciferase reporter assay |
N/A |
N/A |
interaction |
The protein level of MAP1LC3B-II was greatly increased in HUVECs with FLJ11812 overexpression.FLJ11812 could bind with MIR4459 targeting ATG13 (autophagy-related 13), and ATG13 protein level was decreased along with 3BDO-decreased FLJ11812 level. |
25437332 |
|
miRNA chip assay, overexpression, 3BDO |
vascular endothelial cells (VECs) |
N/A |
interaction |
TGFB2-OT1 regulated the levels of 3 microRNAs, MIR3960, MIR4488 and MIR4459. TGFB2-OT1 acted as a competing endogenous RNA, bound to MIR3960, MIR4488 and MIR4459, then regulated the expression of the miRNA targets CERS1 (ceramide synthase 1), NAT8L (N-acetyltransferase 8-like [GCN5-related, putative]), and LARP1 (La ribonucleoprotein domain family, member 1). TGFB2-OT1 increased the LARP1 level, which promoted SQSTM1 (sequestosome 1) expression, NFKB RELA and CASP1 activation, and then production of IL6, IL8 and IL1B in VECs. |
26565952 |
|