LncRNA Information
ID EL1240 Name SPRY4-IT1 Aliases SPRIGHTLY
Species Homo sapiens Chromosome 5 Start site 142317620
End site 142318322 Chain minus Exon NO. 1
Assembly Ensembl Release 89 Class lincRNA NCBI accession NR_131221
Ensembl ENSG00000281881 Sequence

Disease Method Sample Expression pattern Dysfunction type Description PMID Source
melanoma knockdown, affinity purification, mass spectrometry melanoma cells up-regulated expression SPRY4-IT1 knockdown may induce apoptosis via lipin 2-mediated alterations in lipid metabolism leading to cellular lipotoxicity. 25344859
prostate cancer microarray, qPCR, knockdown etc. urine, cell lines (PC3, LNCaP) up-regulated expression We identified a group of differentially expressed long noncoding RNAs in prostate cancer cell lines and patient samples and further characterized six long noncoding RNAs (AK024556, XLOC_007697, LOC100287482, XLOC_005327, XLOC_008559, and XLOC_009911) in prostatic adenocarcinoma tissue samples and compared them with matched normal tissues. Interestingly, these markers were also successfully detetced in patient urine samples and were found to be up-regulated when compared with normal urine. 25513185 Lnc2Cancer
breast cancer microarray, qPCR, Western blot, knockdown etc. breast cancer tissue, cell lines (MD-MB-231, MD-MB-435S, MCF-10A, MCF-7 etc.) up-regulated interaction SPRY4-IT1 expression was significantly upregulated in 48 breast cancer tumor tissues comparedwith normal tissues. Additionally, increased SPRY4-IT1 expression was found to be associated with a larger tumor size and an advanced pathological stage in breast cancer patients. SPRY4-IT1 is a novel prognostic biomarker and a potential therapeutic candidate for breast cancer. 25742952 Lnc2Cancer
melanoma N/A N/A N/A expression The lncRNA, SPRY4-IT1, which is up-regulated in human melanomas compared to melanocytes and keratinocytes, affects cell dynamics, including increased rate of wound closure upon ectopic expression. 22492512 LncRNADisease
melanoma N/A N/A N/A expression Another elegant study by Khaitan et al. utilized a non-coding RNA microarray approach to identify differentially regulated lncRNAs in melanoma cells and identified a 687 bp single exon lncRNA named SPRY4-IT1 which was highly up-regulated in melanoma patient samples and cell lines. 24115003 LncRNADisease
esophageal squamous cell carcinoma qPCR etc. blood (plasma and serum) up-regulated expression Furthermore, plasma levels of POU3F3, HNF1A-AS1 and SPRY4-IT1 were significantly higher in ESCC patients compared with normal controls.By receiver operating characteristic curve (ROC) analysis, among the three lncRNAs investigated, plasma POU3F3 provided the highest diagnostic performance for detection of ESCC (the area under the ROC curve (AUC), 0.842. 25608466 Lnc2Cancer
esophageal squamous cell carcinoma qPCR, knockdown etc. ESCC tissue, cell lines (KYSE-450, KYSE-510, KYSE-150 etc.) up-regulated expression Long?noncoding?RNA?SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. 24810925 LncRNADisease Lnc2Cancer
clear cell renal cell carcinoma qPCR, knockdown etc. ccRCC tissue, cell lines (786-O, ACHN, Caki-1, Caki-2) up-regulated expression The relative level of SPRY4-IT1 was significantly higher in ccRCC tissues compared to the adjacent normal renal tissues. And higher expression of SPRY4-IT1 was found in renal cancer cell lines compared with the normal human proximal tubule epithelial cell line HK-2. The ccRCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. 25337221 Lnc2Cancer
bladder cancer qPCR, knockdown etc. bladder cancer tissue, cell lines (J82, T24, SW780, SV-40 etc.) up-regulated expression SPRY4-IT1 levels were highly positively correlated with histological grade, tumor stage, and lymph node metastasis and reduced overall survival. A multivariate analysis showed that SPRY4-IT1 expression is an independent prognostic factor of overall survival in patients with UCB. 25973088 Lnc2Cancer
melanoma qPCR, knockdown, FISH etc. melanoma tissue up-regulated N/A The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apoptosis and invasion. 21558391 LncRNADisease Lnc2Cancer
gastric cancer qPCR, Western blot, knockdown etc. gastric cancer tissue, cell lines AGS, BGC-823, HGC-27, SGC-7901, MGC80-3, MKN-45) up-regulated N/A SPRY4-IT1 expression was elevated in GC tissues and cell lines 25835973 LncRNADisease Lnc2Cancer
gastric cancer qPCR, Western blot, knockdown etc. gastric cancer tissue, cell lines (SGC7901, BGC823, MGC803, AGS, MKN45 etc.) down-regulated expression SPRY4-IT1 expression is decreased in gastric cancer tissues and associated with larger tumor size, advanced pathological stage, deeper depth of invasion and lymphatic metastasis. Patients with lower SPRY4-IT1 expression had a relatively poor prognosis. DNA methylation may be a key factor in controlling the SPRY4-IT1 expression. Furthermore, SPRY4-IT1 contributed to gastric cancer cells metastasis might partly via regulating epithelial-mesenchymal transition (EMT) process. 26238992 Lnc2Cancer
glioma qPCR, Western blot, knockdown etc. glioma cell lines (U251, SF295) and the normal human astrocytes (NHA) up-regulated expression We examined for the first time the expression and role of SPRY4-IT1 in glioma cells. The results of our study showed that SPRY4-IT1 was up-regulated in human glioma tissues and cell lines. Knockdown of SPRY4-IT1 could inhibit glioma cell growth and migration. Moreover, knockdown of SPRY4-IT1 could inhibit epithelial-mesenchymal transition (EMT) phenotype in glioma cells. 26464658 Lnc2Cancer
non-small-cell lung cancer qRT-PCR patients suffering from non-small-cell lung cancer (NSCLC) up-regulated expression N/A 26448925
melanoma qRT-PCR, WB, knockdown severe preeclamptic placenta, trophoblast cells HTR-8/SVneo up-regulated expression SPRY4-IT1 knockdown enhanced the cell migration and proliferation, and reduced the response of cells to apoptosis. However, exogenous SPRY4-IT1 overexpression significantly decreased the cell migration and proliferation, while increased cell apoptosis. 24223182

Function (not disease relevant)
Methods Sample/condition Expression pattern Dysfunction type Description PMID Source
N/A N/A down-regulated interaction The lncRNA SPRY4-IT1 regulates the intestinal epithelial barrier function by altering expression of tight junction (TJ) proteins. SPRY4-IT1 silencing led to dysfunction of the epithelial barrier in cultured cells by decreasing the stability of mRNAs encoding TJ proteins claudin-1, claudin-3, occludin, and JAM-1 and repressing their translation. In contrast, increasing the levels of SPRY4-IT1 in the intestinal mucosa protected the gut barrier in mice exposed to septic stress by increasing the abundance of TJ proteins. 26678886

Interaction target Level of interaction Type of interaction Description PMID Source
lipin 2 RNA-Protein binding We affinity purified SPRY4-IT1 from melanoma cells and used mass spectrometry to identify the protein lipin 2, an enzyme that converts phosphatidate to diacylglycerol (DAG), as a major binding partner. 25344859
ZNF703 RNA-DNA regulation ZNF703 was a target of SPRY4-IT1 and was downregulated by SPRY4-IT1 knockdown. 25742952
tight junction (TJ) mRNAs RNA-RNA regulation SPRY4-IT1 directly interacted with TJ mRNAs, and this process was enhanced through the association with the RNA-binding protein HuR. 26678886