RT-PCR |
N/A |
N/A |
N/A |
Some were already characterized small RNA precursors, such as the tasiRNA precursor TAS3 (npc41) or the MIR162A precursor (npc78) (Hirsch et al. 2006). The npc83 and npc521 corresponded to the miRNA precursors MIR869A and MIR160C, respectively. Among seven known miRNA precursors spotted on the RIBOCHIP, six (MIR160C, MIR162A, MIR164B, MIR166A, MIR166B, and MIR168A) were up-regulated in dcl1 inflorescences, consistent with DCL1-processed conserved miRNAs. In contrast, the nonconserved MIR869A precursor (npc83) did not overaccumulate in dcl1. This miRNA precursor has an unusually stable secondary structure. |
18997003 |
PLNlncRbase
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