RT-PCR |
dcl1 mutant |
up-regulated |
N/A |
The npcRNA78 gene contains the miR162 sequence in an alternative intron and corresponds to the MIR162a locus. Although DICER-LIKE 1 (DCL1) mRNA is known to be regulated by miR162-guided cleavage, its level does not change in a mir162a mutant. Alternative splicing of npcRNA78 leads to several transcript isoforms, which all accumulate in a dcl1 mutant. This suggests that npcRNA78 is a genuine substrate of DCL1 and that splicing of this microRNA primary transcript and miR162 processing are competitive nuclear events. |
16500993 |
PLNlncRbase
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